Simulation method based on PET image for tail vein extravasation correction / 中华核医学与分子影像杂志

Objective:To establish a method for correction of tail vein extravasation based on PET images and to improve the accuracy of SUV.Methods:The simulation method was established by phantom on Nano PET/CT and images were reconstructed by a three-dimensional ordered-subsets exception maximum algorithm. PET images were analyzed by using the Interview Fusion 1.0 software. The optimal scanning time and the ROI delineated method were found. The accuracy of the simulation method was verified by comparing the activity of simulation method with the mice tail activity measured by the dose calibrator directly on Kunming (KM) mouse ( n=11). Using the simulation method, the impact of extravasation on SUV was proved. Independent-sample t test was used to analyze data. Results:Ten minutes was selected as the optimal scanning time and SUV max 42% threshold was selected as the ROI delineated method. The specific correction formula was as follows: actual activity=image activity/(4.48× V+ 77.05)×100 (0.3 MBq/ml≤leakage concentration<6.5 MBq/ml); actual activity=image activity/(6.65× V+ 71.10)×100 (6.5 MBq/ml<leakage concentration≤14.8 MBq/ml). In the verification experiment, the difference rate between simulated and standard activity was (6.10±2.43)%. In actual operation verification, SUV was underestimated by (6.07±2.67)%. The corrected SUV (0.276±0.078) was not significantly different from the standard SUV (0.277±0.078; t=0.99, P=0.353), while the uncorrected SUV (0.264±0.078) was significantly lower than the standard SUV ( t=7.02, P<0.001). Conclusion:The correction calculation method based on PET images can realize the accurate correction of tail vein leakage activity and the SUV results of animal imaging.

Helicopter emergency medical services in Bayannur: case study / 中华医院管理杂志

Objective:To provide references for aeromedical rescue system development and rational resource allocation for such services, based on analysis of helicopter emergency medical service(HEMS) cases of Bayannur Hospital in Inner Mongolia.Methods:HEMS cases of Bayannur Hospital from 2018 to 2019 were studied, covering such data as patients′ age, gender, disease type, medical service modes, medical service area, and service time. Measurement data were expressed by ± s, and enumeration data were expressed by case number and ratio, while descriptive approach was used for analysis. Results:From 2018 to 2019, 178 patients in Bayannur used HEMS, respectively 39 cases in 2018 and 139 cases in 2019, including 32 cases(17.98%)of pre-hospital emergency care and 146 cases(82.02%) of inter-hospital transfer. The top 3 diseases of the disease spectrum were 73(41.01%) traumatic diseases, 44(24.72%) cardiovascular diseases, and 23(12.92%) neurological diseases.Conclusions:HEMS of Bayannur Hospital has played an important role in emergency medical service for the people in remote areas, and has become an essential method for constructing the air-ground cooperation emergency medical system in West China.

Treatments and prognoses for spinal giant cell tumors recurrence after total spondylectomy / 中华骨科杂志

Objective To investigate the treatment and prognosis of spinal giant cell tumors recurrence after total spondylectomy.Methods Between February 2001 and February 2016,five female patients suffered from spinal giant cell tumors recurrence after total spondylectomy were retrospectively analyzed.They underwent total spondylectomy with an age ranging from 17 to 63 years old (average 32.6 years).Four cases were primary cases and 1 was recurrent case at the first time of visit.Tumors were located in the thoracic vertebrae in 3 cases,including 1 case in T10 and 2 cases in T11.Tumors were involved in lumbar vertebrae in 2 cases,which were L2 and L5,respectively.According to Tomita spinal tumor classification,all cases were type 5.The Frankel classification for spinal cord injury was grade A in 1 case,grade C in 1 case,grade D in 1 case and grade E in 2 cases.Reoperation was introduced to treat the recurrent tumors after total spondylectomy with adjuvant radiotherapy and drug.The effect of tumor therapy,postoperative complications,nerve function and so on were observed in follow-up.Results One patient was treated with total en bloc spondylectomy (TES) and 4 patients was treated with piecemeal total spondylectomy.The operative approach was onestage posterior approach in 4 patients and combined antero-posterior approach in 1 patient.One patient received radiotherapy and 1 patient was treated with bisphosphonate post operation.The spinal cord function recovered to grade D in 1 patient and grade E in 4 patients.The average interval of recurrence after total spondylectomy was 12-57 months (average 29.6 months).At the second visit,the Frankel classification was grade C in 1 patient.Grade D and grade E were in 2 patients,respectively.Three patients were treated with reoperation to resect the tumors.One patient received radiotherapy and another one was untreated.All 5 patients were followed up with 47-196 months (average 108 months).Four patients survived without tumor and 1 patient without treatment for recurrence survived with tumor.Two patients occurred internal fixation fractures were treated with revision surgery.One of the patients had postoperative spinal canal infection and was cured after internal fixation removal,irrigating and drainage,and antibiotic treatment.The loosening screw in 1 patient was fixed again.Two patients suffered from obvious titanium mesh subsidence (＞ 5 mm).In the last follow-up,there were 1 patients with Frankel grade A,1 with grade D and 3 with grade E.Conclusion Active reoperation combined with radiotherapy and drugs therapy can significantly improve the prognosis of patients with recurrent spinal giant cell tumors after total spondylecmy.

Impact of 3-year survival rate on treating ⅡB limb osteosarcoma patients with methotrexate plus DIA chemotherapy regimen / 中华骨科杂志

Objecitve To evaluate the impact of adding methotrexate into DIA chemotherapy regimen (cisplatin,therarubicin and ifosfamide) on 3-year disease-free and overall survival of patients with ⅡB osteosarcoma in extremities.Methods Retrospectively analyzed 34 cases of ⅡB limb osteosarcoma according to the Musculoskeletal Tumor Society (MSTS) surgical staging system with pathologically diagnosed concurrent chemotherapy and comprehensive surgical treatment from May 2008 to April 2014.There were 20 males and 14 females,with an average age of 19.5±7.7 years (ranged from 9 to 44 years).28 patients underwent limb salvage surgery,and 6 patients received amputation.Tumor site included distal femur in 21 patients,proximal tibia in 9 patients,other sites in 4 patients.Seventeen patients underwent cisplatin,THP and ifosfamide (DIA) regimen of chemotherapy.The other 17 patients underwent cisplatin,THP,ifosfamide and methotrexate (MDIA) regimen.Patients charts were reviewed to get demographic data including age,gender,site,tumor size,surgery type,chemotherapy regimen,alkaline phosphatase,lactase dehydrogenase.The prognostic value of these factors on 3 years disease free survival and overall survival were calculated with KaplanMeier method and compared with Log-Rank test between different groups in univariate comparison and COX model for multivariate analysis was used to determine whether these demographic factors are independent prognostic factor for survival Results Two patients had local recurrence,7 patients had metastasis,and 2 patients had both local recurrence and metastasis..The 3-year survival of the whole group was 67.6％ (23/34).Seven patients died during follow-up.The overall survival was 79.4％ (27/34).The 3 -year disease-free survival in DIA group and MDIA group was 70.6％ (12/17) and 64.7％ (11/17),respectively.The 3-year overall survival was 82.4％ (14/17) and 76.5％ (13/17) in DIA group and MDIA group,respectively.Univariate analysis showed that there was no significant difference in either 3-year disease-free survival (x2=0.113,P=0.737) or overall survival (x2=0.197,P=0.657) between DIA and MDIA.Age was significantly correlated with prognostic in univariate analysis (x2=5.869,P=O.015),while gender,tumor site,tumor size,surgery type,ALP,LDH was not correlated with disease-free survival.Kaplan-meier analysis with LogRank test showed age (x2=6.134,P=O.013) and tumor size (x2=5.108,P=0.024) were statistically correlated with overall survival.Multivariate analysis with COX model showed that chemotherapy regimen was not an independent prognostic factor for 3-year disease-free survival or overall survival.Age was an independent prognostic factor for 3-year disease-free survival (HR=5.836,P=0.017).Conclusion The addition of MTX on the basis of DIA chemotherapy regimen did not significantly improve the 3-year disease-free survival and overall survival of stage ⅡB limb osteosarcoma.

Roles and regulation mechanism of microRNA-218 in acute lymphocytic leukemia cell CCRF-CEM / 中国免疫学杂志

Objective:To detect the expression of microRNA-218 (miR-218) in human acute lymphocyte leukemia (ALL) T lymphocytes ( CCRF-CEM) ,explore its effects on the biological features of CCRF-CEM cells and the expression of its target gene c-kit, so as to provide new insights for leukemia treatment.Methods: Using the quantitative real-time polymerase chain reaction ( qRT-PCR) ,we detected the expression of miR-218 in the normal peripheral blood T lymphocytes and CCRF-CEM cells.Forty-eight hours after the miR-218 mimic was transfected into the CCRF-CEM cells,the expression of miR-218 in the CCRF-CEM cells was detected by qRT-PCR.The effect of miR-218 on the CCRF-CEM cell viability was detected using MTT.The effect of miR-218 on the proliferation and apoptosis of CCRF-CEM cell was analyzed using flow cytometry.c-kit gene was identified to be a target gene of miR-218 by luciferase reporter enzyme system,and the effect of miR-218 on the expression of KIT protein in cells were determined using Western blot.Results:As shown by qRT-PCR,compared with that in the normal peripheral blood T lymphocytes,the expressions of miR-218 in ALL T lymphocytes cell lines were significantly decreased ( P<0.01 ) .Compared with the control group, the expression of miR-218 increase significantly in CCRF-CEM cells transfected with miR-218 mimic for 48 hours ( P<0.01).MTT showed that the cell viability decreased significantly after the over-expression of miR-218 in the CCRF-CEM cells ( P<0.05 ) .Flow cytometry showed that the S-phase fraction significantly declined after the over-expression of miR-218 ( P<0.01 ) , and meanwhile the apoptosis of cells also significantly increased (P<0.01).Luciferase reporter gene assay showed that,compared with the control group,the relative luciferase activity significantly declined in the miR-218 mimic transfection group (P<0.01).Compared with the control group,the expression of KIT protein in the CCRF-CEM cells transfected with miR-218 mimic for 48 hours significantly decreased ( P<0.01).Conclusion:The expression of miR-218 decreases in ALL T lymphocytes cell lines.MiR-218 can negatively regulate the expression of KIT protein,inhibit the proliferation and increase the apoptosis of CCRF-CEM cells.Treatment based on the enhanced expression of miR-218 may be a promising strategy for leukemia.

Limited revision for extramedullary stem fracture of the custom articular prostheses / 中华骨科杂志

Objective To introduce a new method of limited revision for the extramedullary stem fracture of the custommade tumor prostheses and evaluate its clinical effect.Methods There were 3 patients with extramedullary stem fracture after custom-made tumor prostheses replacement,2 male and 1 female.The age was 25,51 and 52 years old respectively.The pathological diagnosis and involved locations were osteosarcoma of distal femur,recurrent giant cell tumor of distal femur,and chondrosarcoma of proximal femur.The interval between replacement operation and extramedullary stem fracture were 11 months,34 months and 28 months respectively.The fracture occurred when they were walking without obvious history of trauma.The fracture sites located at the conjunction of the extramedullary stems in distal femoral prostheses and femoral neck basis in proximal femoral prosthesis.According to the firm fixation of the intramedullary stem and the enough length of the residual extramedullary stem,the revision prosthesis for limited revision operation was designed in order to avoid removing the firmly fixed intramedullary stem and bone cement which is very difficult.The revision prosthesis consists of sleeve part and joint part with the same material as former prosthesis,which is sleeved to the residual extramedullary stem and fixed by bone cement and interference screws in limited revision operation.The wearing fittings were replaced at the same time.Patients took regularly functional exercises postoperatively.The stability of revision prosthesis and the function of the involved limbs were observed in follow-up.Results The reasons of prosthesis fracture were fatigue fractures at the conjunction of extramedullary stem in one distal femur prosthesis,at the basis of femoral neck in one proximal femur prosthesis and conjunction loosening and locked screw breakage in the other distal femur prosthesis.The follow-up times were 1 month,103 months and 1 10 months respectively.One patient with giant cell tumor underwent tumor resection due to tumor recurrence in soft tissue.The revision prostheses were fixed firmly without loosening.According the MSTS scoring system,the scores of involved extremities function were 66.7％、86.7％ and 83.3％ respectively.Conclusion The fracture of custom-made tumor prosthesis could occur due to metal fatigue and conjunction failure.The newly designed revision prosthesis allows a limited revision operation with preserving the firmly fixed intramedullary stem,which reduces the difficulty of operation and makes the recovery of the limb function as soon as possible.

Expression and significance of BAFF/APRIL in childhood acute lymphoblastic leu-kemia / 中国免疫学杂志

Objective:To investigate the expression and significance of B cell activating factor (BAFF) and a proliferation-inducing ligand ( APRIL) in children with acute lymphoblastic leukemia ( ALL).Methods:The mRNA and protein expressions in ALL.

GPI-PLD inhibits the growth of hepatoma cells by down-regulation of PI3K-Akt signaling pathway / 中南大学学报(医学版)

OBJECTIVE@#To clarify the effect of glycosylphosphatidylinositol-specific phospholipase D (GPIPLD) on hepatoma cells HepG2 and the possible molecular mechanism.@*METHODS@#MTT, fluorescent staining and Western blot were applied to analyze the effect and molecular mechanism of GPI-PLD on hepatoma cells by transfected high expression GPI-PLD model. We inoculated HepG2 in nude mice models to further clarify the effect of GPI-PLD on hepatoma cells in vivo.@*RESULTS@#Compared with the control groups, PI3K-Akt signaling pathway activity and proliferation of hepatoma cells were significantly inhibited in the GPI-PLD group. Nude mice models showed that the tumor growth and tumor weight [(1.87 ± 0.09) g] of the GPI-PLD group were significantly less than those of the blank control group [(2.20 ± 0.17) g] and the negative control group [(2.15 ± 0.09) g]. AST, ALT and AFP serum concentration in the GPI-PLD group were significantly lower than those of the control groups (P<0.05).@*CONCLUSION@#GPI-PLD can inhibit the proliferation of hepatoma cells and growth in vivo, and promote the apoptosis of hepatoma cells by reducing the activity of PI3K-Akt signaling pathway.

Anatomical research and clinical application of extraarticular total knee resection / 中华骨科杂志

Objective To investigate technique of reservation and reconstruction of the extensor mechanism in extraarticular total knee resection.Methods There were 6 cases of fresh total knee joint specimen for anatomical observation.After meglumine diatrizoate was injected into the joint capsule and the deep infrapatellar bursa,the CT examination of the knee specimen was performed.The specimens were detached according to the requirement of extraarticular knee resection,after methylene blue was injected into the joints capsule.The relationships between articular capsule,infrapatellar fat pad and deep infrapatellar bursa were observed,as well as feasibility of extraarticular total knee resection.Four patients with malignant bone tumor around the knee were performed extraarticalar total knee resection.The patellar ligament was reserved completely in 2 patients whose infrapatellar fat pads were not be involved by tumor confirmed by deep infrapatellar bursa radiography.However,the patellar ligament was split along the coronal plane in 2patients who had not undergone deep infrapatellar bursa radiography.The patients were followed up to observe results of tumor control and function of the affected knees.Results Through anatomical observation,some bursas around the knee joints,such as the suprapatellar bursa and popliteal bursa,communicated with the joint cavity,while the deep infrapatellar bursa did not.Furthermore,the infrapatellar fat pad located between the patellar tendon and joint cavity to form a good barrier to tumor invasion.So the patellar tendon could be reserved completely if the infrapatellar fat pad was not involved.Four patients were followed up for 10 to 28 months (average,19 months).At final follow-up,there was no local recurrence and metastasis in all 4 patients.The range of flexion of the affected knees ranged from 95° to 118 ° (average,105 °),and the average extension lag was 22 ° (range,10° to 40 °).The MSTS score was 72.5％.Conclusion For patients with malignant bone tumor involving the knee,the deep infrapatellar bursa radiography should be performed routinely.The patellar ligament could be reserved completely in the extraarticular total knee resection when the deep infrapatellar bursa doesn't communicate with the joint cavity combined with presence of the infrapatellar fat pad.Sometimes,only reserving the superficial half of the patellar ligament also could meet the need of extension.The short-term result of tumor control and function of the affected knee are satisfactory.

Stability reconstruction and related complications after total spondylectomy for thoracolumbar tumors / 中华骨科杂志

Objective To investigate the spinal stability reconstruction and related complications after total spondylectomy for thoracolumbar tumors. Methods From January 1997 to December 2009, 34 cases with thoracolumbar tumors treated with total spondylectomy, including 20 males and 14 females with an average age of 43 years. The tumors were located in thoracic vertebra in 27 cases and lumbar vertebra in 7, including 6 spinal metastases and 28 primary tumors. The total spondylectomy was piecemeal in 23 cases and en bloc in 11. The reconstruction methods included posterior short-segment fixation in 19 cases, multi-segmental fixation in 13, anterior intervertebral fixation in 2, titanium mesh with auto-bone graft in 20 cases, titanium mesh with bone cement in 7, bone cement filling in 2, auto-bone strut graft in 2, titanium mesh with strengthened rings in 1, artificial vertebral body replacement in 2, posterior massive bone bridging graft in 8 and bone particles graft in 26. Results The mean follow-up time was 43 months. Seven patients died, 5 suffered recurrence. Poor grafted bone fusion was found in 5 cases. The intervertebral sagittal Cobb angle of adjacent vertebra was ??after operation, 9.3?at the end of follow-up or before revision operation. The average lost angle was 14.1?for cases with multi-segmental fixation, and 21.5?with short-segment fixation. Complications included internal fixation broken (5) and loosening (2), titanium mesh subsidence (6) and displacement (3). There were 5 revision surgeries. Conclusion There are relative more complications about spinal stability reconstruction after total spondylectomy. To achieve temporary stiff fixation and long-term fusion, the posterior multi-segment fixation and massive bone bridging graft combined with the anterior artificial vertebral body replacement are effective.

Survival and complications in total knee replacement with custom-prosthesis after bone tumors resection / 中华骨科杂志

Objective To retrospectively investigate the endoprosthetic survival and complications after custom-made tumor prosthesis replacement of knee joint.Methods From April 1996 to April 2007,85 patients with bone tumors around knee joints undergoing custom-prosthetic replacement were respectively analyzed,including 54 males and 31 females with an average of 31.33±15.3 years(range,11-72).The diagnoses were osteosarcoma(43 patients),giant cell tumor(31),chondrosarcoma(4),metastatic tumor(3),malignant fibrohistiocytoma(2),Ewing sarcoma(1),and fibrosarcoma(1).The distal femar was affected in 43 patients and the proximal tibia in 42.All patients in this group underwent wide resection and domestic custommade cemented endoprosthetic reconstruction.Results Mean following was 45.8 months.The overall complication rate was 27.1%(23/85),which was 25.6% in distal femar and 28.6% in proximal tibia.Thirteen complications occurred within 3 years after definite surgery,3 in 3-5 years,and 7 over 5 years.All of 6 operation-relation complications(wound dehiscence,periprosthetic infection,peroneal nerve injury,etc) occurred within 3 years.Seven prosthetic-related complications(prosthetic loosening or breaking,dislocation,periprosthetic fracture,etc)occurred within 3 years,3 occurred in 3-5 years,and 7 over 5 years after definite surgery.The overall endoprosthetic cumulative survival rate was 79.4%,67.7%and 62.3% after 3,5 and 7 years,respectively;which was 86.6%,79.2% and 73.5% in distal femur;66.1%,53.7% and 48.8% in proximal tibia.Conclusion All operation-related complications occurred within 3 years,prosthesis-related complications occurred mainly within 3 years and over 5 years postoperatively.Prosthesis-related complications could occur iu the early stage after using domestic custom-made prosthesis reconstruction.

Total en bloc spondylectomy for tumor of the thoracolumbar spine / 中国矫形外科杂志

[Objective]To evaluate the possibility and clinical value of total en bloc spondylectomy for the treatment of spinal tumors. [Method]Four cases of primary tumor of the thoracolumbar spine were treated with total en bloc spondylectomy TES through a single posterior approach performed in three cases(T10 solitary plasmacytoma,T8 hemangioma,and T12 hemangioma),and one case through a single stage anterior and posterior combined approach(L4 neuroblastoma with paraspinal mass).Improvements in osteotomy apparatus and surgical technique of TES were investigated.[Result]All patients attained significant clinical improvement after surgery without any severe complications.Patients were observed for 13 to 22 months.There was no local recurrence and distal metastasis by the last follow-up.[Conclusion]Total en bloc spondylectomy includes resection of the involved vertebra in two major blocs,rather than a piecemeal pattern.It is one of the most effective therapies for spinal tumors.Howerer,some components of TES remain to be further improved.

EFFECT OF PHYTIC ACID ON EXPRESSIONS OF APOPTOSIS ASSOCIATED PROTEIN FAS、BAX AND CASPASE-3 IN SGC-7901 CELLS / 营养学报

Objective:To explore the effect of phytic acid on apoptosis of SGC-7901 cells and its mechanisms. Method:The inhibiting action of phytic acid on SGC-7901 cells was examed by MTT assay,and the effect on apoptosis of SGC-7901 cells was examined by DNA gel electrophoresis,and the expressions of Fas,Bax and caspase-3 protein were detected by Western blot method. Results:From MTT assay,we observed that phytic acid inhibited the growth of SGC-7901 cells in dose and time dependent manner. Typical DNA ladder was found during gel eletrophoresis. Caspase-3 can be activated by phytic acid and the expressions of Bax and Fas protein in phytic acid group were higher compared with control group,and increased in a dose-dependent manner. Conclusion:Phytic acid can induce apoptosis of SGC-7901 cells which may be correlated to apoptosis associated protein Fas,Bax and caspase-3.

Blood lead level of children in the urban areas in China / 中华流行病学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to investigate the blood lead level of 3 - 5 year old children living in the cities in China and to provide scientific data for making policy on environmental lead pollution for children health protection.</p><p><b>METHODS</b>Six thousand five hundred and two vein blood samples from 3 - 5 year old children in nineteen cities of nine provinces were sampled. Inductively coupled plasma-mass spectrometry (ICP-MS) were employed to determine lead level in whole blood after microwave digestion for sample preparation and questionnaire survey was also performed. Data were analyzed with multiple regression on factors which affecting blood lead levels.</p><p><b>RESULTS</b>Results showed that mean blood lead level was 88.3 micro g/L for 3 - 5 year old children living in the cities in China and mean blood lead level of boys (91.1 micro g/L) was higher than that of girls (87.3 micro g/L). Twenty-nine point nine one per cent of the children's blood lead level exceeded 100 micro g/L. The research finding showed: (1) higher blood lead levels had negative effects on children's physical growth, language ability etc. (2) behavior of parents had certain effects on children's blood lead levels. (3) blood lead levels of children were affected by unhealthy habits.</p><p><b>CONCLUSIONS</b>Problem of childhood lead poisoning in China has become more serious. During the past ten years, blood lead levels of children has been increased in China while decreasing in developed countries. Blood lead levels of children in China are higher than that of developed countries, which called for special concern by government and society.</p>

Clinical analysis of 16 cases with olfactory neuroblastoma / 中国癌症杂志

Purpose:To analyze retrospectively the clinical manifestation, treatment and prognosis. Methods:From 1978 to 1996,16 patients with olfactory neuroblastoma were treated.4 cases were stage A,6 cases were stage B and 6 cases were stage C. Six patients were treated with radiotherapy alone or combined with chemotherapy, 9, with surgery and postoperative radiotherapy and 1,with operative alone. The radiation dosage ranged from 40～70Gy with 2 Gy per day. Results:The 1 , 3 and 5 year survival rates were 75%, 75% and 69%.Conclusions:Olfactory neuroblastoma is sensitivity for radiotherapy, for the patients with stage A, radiotherapy alone is effective for stage B and C olfactory neuroblastoma , combined modality of surgery and postoperative radiotherapy should be the treatment of choice.

Toxicity of Algae in Water of Chaohu Lake and Its Influence on Drinking Water Quality / 环境与健康杂志

Objective To explore the toxicity of algae in water of Chaohu Lake and its influence on the quality of drinking water.Methods In1999,the water samples were collected from the sampling location of A point as a discarded water source for Chaohu Water Plant 1.5km far from the lakeside of Chaohu Lake and the sampling location of B point as a water source in use for Chaohu Water Plant 3.0km far from the lakeside of Chaohu Lake.The species and count of algae in these water samples were analyzed.The determination of micro cystin,sanitary indexes of water quality,Ames test,micronucleus test and SCGE test were carried out in the water samples from B point and the finished water from water plants using water source for B point.Re -sults83species of algae belong to8phylum and49genus were found in water samples from A point and B point in Chao hu Lake.The anual averages of algae count in water sample of B point (8205.67?10 4 /L)exceeded16.8%of that of A point water samples(7024.27?10 4 /L).Microcystin was found in12water samples among total16water samples collected from the lake water,two of which revealed excessive content s of microcystin exceeding standard,but it was not found in raw water and finished water samples from five plants in Hefei and Chaohu Lake[except the4th water plant (4th WP )].After transportation and stor-age,the raw water of4th WP of Hefei with lower content s of microcystin revealed higher contents of microcystin exceeding the standard in primary finished water samples of clean-well finished water storage,but it couldnt be found in secondary finished water samples with the processes of coagulation,ch lorination and active carbon-filtration.The total nitrogen and other6indexes of raw water of4th WP exceeded the related standards ruled by GB3838-2002Surface Water Environment Standard Grade V in different degree,but above6water quality indexes could reach the related sanitary standard after water treatment.Ames test and micronucleus test indicated that the raw water of4th WP presented suspectable mutagenicities which couldnt be removed by co-agulation and chlorination.SCEG test also showed that the organic extracts of the raw water of4th WP presented DNA damage effects.Con clusion The water source in use in Chaohu Lake revealed heavier pollution by algae compared with the original discarded water source in Chaohu Lake.Chaohu Lake water and its finished water revealed higher levels of microcystin exceed-ing the standard and the organic extracts from raw water and finished water presented suspectable mutagenicity.

Investigation on Water Pollution by Algae at Locations of Water Collection in Chaohu Lake / 环境与健康杂志

winter. The dominant species of algae were Microcystis aeruginosa, Anabaena spiroides and Spirulina . The annual average contents of total nitrogen, total phosphorus and chlorophyl-a were 4. 30 mg/ L, 0.09 mg/ L and 0.015 mg/ L respectively, all of which exceeded the related temporary standards in China. Conclusion The source water of Chaohu Lake had been in the state of serious eutrophication.

Effects of Ternatolide on expression granulysin mRNAin the peripheral blood lymphocytes patients with mycobacterium tuberculosis / 中国药理学通报

AIM To study the effects of ternatolide(tern) on expression of granulysin mRNA in the activated peripheral blood lymphocytes(PBLS) of the adults and children with pulmonary Mycobacterium tuberculosis(PMTB). METHODS Using competitive quantitative reverse transcription polymerase chain reaction(QC TR PCR)analyze granulysin gene expression in the PBLs. RESULTS The dosage of 100 mg?L -1 , 200mg?L -1 of ternatolide significantly enhanced the granulysin mRNA experession in PBLs in vitro stimulated phytohemagglutinin (PHA) from adults and children with PMTB (P0 05). CONCLUSION The molecular mechanism of tern. against intracellular pathogens might be associated with the inducting highly level on the expression of granulysin mRNA, a anti bacterial peptide in activated human cytotoxic lymphocytes.

STUDY ON ROLE OF C-MYC IN APOPTOSIS OF HUMAN GASTRIC CARCINOMA CELLS INDUCED BY PHYTIC ACID / 营养学报

Objective To explore the effect of phytic acid on apoptosis of human gastric carcinoma SGC-7901 cells and its mechanism. Method The inhibiting action of phytic acid on SGC-7901 cells was examined by MTT assay; the morphology alteration of SGC-7901 cells was examined by reverse discrepancy microscope; and the expression of c-myc protein was detected by immunohistochemisty method and Western blot method. Results Phytic acid inhibited the growth of human gastric carcinoma SGC-7901 cells in dose and time dependent manner. The growth of cells in test groups were inhibited higher than in control group. The expression of c-myc protein in phytic acid group was lower compared with control group,and reduced in a dose-dependent manner. Conclusion Phytic acid can induce apoptosis of human gastric carcinoma SGC-7901 cells,and the mechanism may be related to apoptosis associated gene c-myc.